- UV-vis
UV-vis
Ultraviolet–visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis)
refers to absorption spectroscopy or reflectance
spectroscopy in the ultraviolet-visible spectral region.
This means it uses light in the visible and adjacent (near-UV and near-infrared (NIR)) ranges. The absorption or reflectance in the visible
range directly affects the perceived color
of the chemicals involved. In this region of the electromagnetic spectrum, molecules undergo electronic
transitions. This technique is complementary to fluorescence spectroscopy, in that fluorescence deals with transitions from the excited state to the ground
state, while absorption measures transitions from the
ground state to the excited state.
